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Registros recuperados: 992 | |
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Rodríguez,Esteban M; Guerra,María M; Vío,Karin; González,César; Ortloff,Alexander; Bátiz,Luis F; Rodríguez,Sara; Jara,María C; Muñoz,Rosa I; Ortega,Eduardo; Jaque,Jaime; Guerra,Francisco; Sival,Deborah A; Dunnen,Wilfred F. A. den; Jiménez,Antonio J; Domínguez-Pinos,María D; Pérez-Fígares,José M; McAllister,James P; Johanson,Conrad. |
Most cells of the developing mammalian brain derive from the ventricular (VZ) and the subventricular (SVZ) zones. The VZ is formed by the multipotent radial glia/neural stem cells (NSCs) while the SVZ harbors the rapidly proliferative neural precursor cells (NPCs). Evidence from human and animal models indicates that the common history of hydrocephalus and brain maldevelopment starts early in embryonic life with disruption of the VZ and SVZ. We propose that a "cell junction pathology" involving adherent and gap junctions is a final common outcome of a wide range of gene mutations resulting in proteins abnormally expressed by the VZ cells undergoing disruption. Disruption of the VZ during fetal development implies the loss of NSCs whereas VZ disruption... |
Tipo: Journal article |
Palavras-chave: Hydrocephalus; Abnormal neurogenesis; Neural stem cells; Stem cell transplantation. |
Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000300005 |
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Deniz Koç,Nazan; Yüce,Rikap. |
In sexually reproducing organisms, primordial germ cells (PGCs) give rise to the cells of the germ line, the gametes. In many animals, PGCs are set apart from somatic cells early during embryogenesis. This study explores the origin of primordial germ cells (PGCs) of the zebra fish and examines their morphology during early development (1st day-15th day). PGCs were selectively stained by the alkaline phosphatase histochemical reaction and viewed by light and electron microscopy from the time they are first detectable in the yolk sac endoderm. PGCs occurred in the subendodermal space on the syncytial periblast; differing from the surrounding endodermal cells. Later the PGCs moved to between the blastoderm and yolk sac and transferred to the dorsal mesentery... |
Tipo: Journal article |
Palavras-chave: Primordial germ cell; Zebra fish; Ultrastructure; Morphology. |
Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000400001 |
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Marçal-Pessoa,Ana Flávia; Bassi-Branco,Carmen Lucia; Salvatierra,Cristiana dos Santos Barbosa; Stoppiglia,Luiz Fabrizio; Ignacio-Souza,Letícia Martins; Reis,Sílvia Regina de Lima; Veloso,Roberto Vilela; Reis,Marise Auxiliadora de Barros; Carneiro,Everardo Magalhães; Boschero,Antonio Carlos; Arantes,Vanessa Cristina; Latorraca,Márcia Queiroz. |
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Tipo: Journal article |
Palavras-chave: Connexin 36 Connexin 43 β -catenin Low protein diet Pregnancy Pancreatic islets Rat. |
Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100003 |
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RIVAS,MARIELLA; SEEGER,MICHAEL; HOLMES,DAVID S; JEDLICKI,EUGENIA. |
The genome of the acidophilic, proteobacterium Acidithiobacillus ferrooxidans, contains linked but divergently oriented genes, termed afeI and afeR, whose predicted protein products are significantly similar to the LuxI and LuxR families of proteins. A possible promoter and Lux box are predicted upstream of afeI. A cloned copy of afeI, expressed in E. coli, encodes an enzyme that catalyzes the production of a diffusible compound identified by gas chromatography and mass spectrometry as an unsubstituted N-acyl homoserine lactone (AHL) of chain length C14. This AHL can be detected by a reporter strain of Sinorhizobium meliloti Rm41 suggesting that it is biologically active. The reporter strain also responds to extracts of the supernatant of A. ferrooxidans... |
Tipo: Journal article |
Palavras-chave: A. ferrooxidans; Quorum-sensing; LuxI and LuxR; Lux box; Homoserine lactone. |
Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000200018 |
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LE PROVOST,GREGOIRE; HERRERA,RAÚL; PAIVA,JORGE AP; CHAUMEIL,PHILIPPE; SALIN,FRANCK; PLOMION,CHRISTOPHE. |
A large quantity of high quality RNA is often required in the analysis of gene expression. However, RNA extraction from samples taken from woody plants is generally complex, and represents the main limitation to study gene expression, particularly in refractory species like conifers. Standard RNA extraction protocols are available but they are highly time consuming, and not adapted to large scale extraction. Here we present a high-throughput RNA extraction protocol. This protocol was adapted to a micro-scale by modifying the classical cetyltrimethylammonium (CTAB) protocol developed for pine: (i) quantity of material used (100-200 mg of sample), (ii) disruption of samples in microtube using a mechanical tissue disrupter, and (iii) the use of SSTE buffer.... |
Tipo: Journal article |
Palavras-chave: RNA extraction; Micromethod; Forest trees. |
Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000400003 |
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HAMAME,CARLOS M; DELANO,PAÚL H; ROBLES,LUIS. |
Twelve rats were trained to perform a two-choice visual detection task in which a right or left light was presented and the animáis were required to press the lever located under the illuminated light for a food reward. In seventy percent of the triáis the target light was preceded by presentation of a neutral cue (a central light). Relevance of the neutral cue for detection of the target was analyzed by comparing behavioral Índices of attention in its presence and absence. Accuracy was significantly higher in presence than in absence of the neutral cue, while mean response latencies were lower in presence than in absence of the neutral cue. These results indicate that the animáis allocated attentional resources on the target detection during a high... |
Tipo: Journal article |
Palavras-chave: Alerting effect; Attention; Detection task; Expectancy; Neutral cue; Rat. |
Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000400012 |
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GONZÁLEZ,RODRIGO; TIRADO,ARES; BALANDA,MONSERRAT; ALVO,MIRIAM; BARQUÍN,INÉS; DURRUTY,PILAR; LOBOS,SERGIO; SEELENFREUND,DANIELA. |
Diabetic nephropathy (DN) is one of the major complications of type 2 diabetes and is associated with coronary disease. Nephrin, a protein mainly expressed in glomeruli, is decreased in DN and other kidney diseases. Since insulin levels are misregulated in type 2 diabetes, a possible connection between DN and its decreased nephrin expression could be the presence of regulatory elements responsive to insulin in the nephrin gene (NPHS1) promoter region. In this work, using bioinformatic tools, we identified a purine-rich GAGA element in the nephrin gene promoter and conducted a genomic study in search of the presence of polymorphisms in this element and its possible association with DN in type 2 diabetic patients. We amplified and sequenced a 514 bp promoter... |
Tipo: Journal article |
Palavras-chave: GAGA box; Gene promoter; Nephrin; Polymorphism. |
Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000400010 |
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Shen,Wenlong; Wang,Dong; Ye,Bingyu; Shi,Minglei; Zhang,Yan; Zhao,Zhihu. |
BACKGROUND: The CCCTC-binding factor (CTCF) is a highly conserved insulator protein that plays various roles in many cellular processes. CTCF is one of the main architecture proteins in higher eukaryotes, and in combination with other architecture proteins and regulators, also shapes the three-dimensional organization of a genome. Experiments show CTCF partially remains associated with chromatin during mitosis. However, the role of CTCF in the maintenance and propagation of genome architectures throughout the cell cycle remains elusive. RESULTS: We performed a comprehensive bioinformatics analysis on public datasets of Drosophila CTCF (dCTCF). We characterized dCTCF-binding sites according to their occupancy status during the cell cycle, and identified... |
Tipo: Journal article |
Palavras-chave: DCTCF; Cell cycle; Chromatin domains; Mitotic bookmarking; Bioinformatics. |
Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100027 |
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CANALS,MAURICIO; OLIVARES,RICARDO; ROSENMANN,Mario. |
In this paper we develop a method to estimate lung volume using chest x-rays of small mammals. We applied this method to assess the lung volume of several rodents. We showed that a good estimator of the lung volume is: V*L = 0.496 · V RX ≈ 1/2·V RX , where V RX is a measurement obtained from the x-ray that represents the volume of a rectangular box containing the lungs and mediastinum organs. The proposed formula may be interpreted as the volume of an ellipsoid formed by both lungs joined at their bases. When that relationship was used to estimate lung volume, values similar to those expected from allometric relationship were found in four rodents. In two others, M. musculus and R. norvegicus, lung volume was similar to reported data, although... |
Tipo: Journal article |
Palavras-chave: Lung volume; X-ray; Method; Rodents. |
Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100006 |
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MEISEL,LEE; FONSECA,BEATRIZ; GONZÁLEZ,SUSANA; BAEZA-YATES,RICARDO; CAMBIAZO,VERONICA; CAMPOS,REINALDO; GONZALEZ,MAURICIO; ORELLANA,ARIEL; RETAMALES,JULIO; SILVA,HERMAN. |
Prunus persica has been proposed as a genomic model for deciduous trees and the Rosaceae family. Optimized protocols for RNA isolation are necessary to further advance studies in this model species such that functional genomics analyses may be performed. Here we present an optimized protocol to rapidly and efficiently purify high quality total RNA from peach fruits (Prunus persica). Isolating high-quality RNA from fruit tissue is often difficult due to large quantities of polysaccharides and polyphenolic compounds that accumulate in this tissue and co-purify with the RNA. Here we demonstrate that a modified version of the method used to isolate RNA from pine trees and the woody plant Cinnamomun tenuipilum is ideal for isolating high quality RNA from the... |
Tipo: Journal article |
Palavras-chave: CDNA library; Fruit; Fruit trees; Functional genomics; Peach; Polysaccharides; Prunus persica; RNA isolation; Rosaceae; RT-PCR. |
Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100010 |
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Registros recuperados: 992 | |
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